本文报道了幽门螺杆菌在２种培养基和４种温度下保存效果的实验研究．１０株被试菌株经改良Ｓｋｉｒｒｏｗ琼脂平板培养２４～４８ｈ，刮取菌苔于含１０％小牛血清、Ｎ％甘油布氏肉汤，在─７０℃条件下能存活１２个月，用同样方法在─４ｏ℃下多数菌株只能存活半个月．将菌苔置于特殊半固体培养基于３５℃传代培养保存，１年存活率为６０％，但在３个月内形态较典型．对存活菌株进行细菌形态，菌落特征和生化特性试验证明保存前后其生物学性状完全一致． This article reports the experimental study of Helicobacter pylori preservation in two media and four temperatures. Ten strains were cultured on modified Skirrow agar plates for 24-48h. The bacteria were scraped in 10% fetal bovine serum and N% glycerol-buchny broth and survived for 12 months at -70 ℃. Using the same method Most strains can survive for only half a month at -4 ° C. The lawn was placed in a special semi-solid culture subculture at 35 ℃, 1-year survival rate was 60%, but within 3 months more typical morphology. Bacterial morphology, colony characteristics and biochemical characteristics of the surviving strains tested before and after the preservation of their biological characteristics of the same.