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目的通过检测C35蛋白在乳腺癌及正常乳腺组织中的表达,揭示C35蛋白在乳腺癌发生、发展、侵袭转移过程中的作用和意义。方法收集68例女性乳腺癌组织及20例乳腺正常组织,应用免疫组化技术检测分析C35蛋白在乳腺癌组织中的表达及其与乳腺癌临床病理特征的关系;分析乳腺癌组织C35蛋白表达与ER、PR、HER-2蛋白表达的相关性及与肿瘤不同分子亚型的关系。结果 1)乳腺癌组织中C35蛋白的表达显著高于正常乳腺组织(P<0.05),而在正常乳腺组织C35蛋白呈现低表达或不表达。C35蛋白表达与乳腺癌组织临床病理学特点存在相关性,随着乳腺癌组织的临床TNM分期、病理组织学分级的增加,C35蛋白阳性率显著升高(P<0.05);2)乳腺癌组织C35蛋白表达与ER与PR的表达呈负相关(r分别为-0.035和-0.083),与HER-2蛋白的表达呈正相关(r=0.207);3)C35在乳腺癌不同分子亚型中的阳性率差异无统计学意义(P>0.05),有无淋巴结转移者C35蛋白阳性率差异有统计学意义(P<0.05)。结论 C35蛋白在乳腺癌细胞中广泛存在,并与乳腺癌的发生、发展及预后有关,因而可作为乳腺癌诊断和治疗的标志物或新靶点。
Objective To detect the expression of C35 protein in breast cancer and normal breast tissues to reveal the role and significance of C35 protein in the occurrence, development, invasion and metastasis of breast cancer. Methods 68 cases of breast cancer tissues and 20 cases of normal breast tissues were collected. The expression of C35 protein in breast cancer tissues and their clinicopathological features were analyzed by immunohistochemistry. The expressions of C35 protein in breast cancer tissues and Correlation of ER, PR, HER-2 protein expression with tumor subtypes. Results 1) The expression of C35 protein in breast cancer tissues was significantly higher than that in normal breast tissues (P <0.05), while the expression of C35 protein in normal breast tissues was low or not expressed. The positive rate of C35 protein in breast cancer was significantly higher than that in breast cancer (P <0.05). The positive rate of C35 protein in breast cancer was significantly higher than that in breast cancer The expression of C35 was negatively correlated with the expression of ER and PR (r = -0.035 and -0.083, respectively), which was positively correlated with the expression of HER-2 protein (r = 0.207). 3) The expression of C35 in different molecular subtypes of breast cancer There was no significant difference in positive rate (P> 0.05). There was significant difference in the positive rate of C35 protein between patients with and without lymph node metastasis (P <0.05). Conclusion The C35 protein is widely found in breast cancer cells and is associated with the occurrence, development and prognosis of breast cancer. Therefore, it can be used as a marker or a new target for the diagnosis and treatment of breast cancer.