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目的 :探讨建立基底动脉栓塞动物模型的方法。方法 :结扎实验豚鼠双侧颈动脉 ,运用4 0 %三氯化铁 (FeCl3)溶液诱导基底动脉栓塞 ,采用激光多普勒流量仪测量诱导栓塞前后耳蜗血流量 (CBF)的变化 ,以压力传感器和前置放大器等装置同步监测平均动脉压 (MABP)的变化。结果 :4 0 %FeCl3溶液诱导 5～ 10min ,CBF开始下降 ,随着诱导时间的延长 ,CBF下降进一步加大 ,诱导 5 0min时 ,CBF下降达 (6 4.5± 4 .2 ) % ,与诱导前比较 ,差异有极显著性意义 (P <0 .0 1)。整个实验期 ,CBF的均值为 (81.3± 12 .0 ) % ,与诱导前比较 ,差异亦有极显著性意义 (P <0 .0 1)。MABP无明显变化。结论 :运用 4 0 %FeCl3溶液能诱导豚鼠基底动脉栓塞 ,为临床动脉栓塞所致内耳微循环障碍机制的研究及抗栓药物的开发应用提供一个理想的实验动物模型
Objective: To explore the method of establishing animal model of basilar artery embolism. Methods: Bilateral carotid arteries were ligated and basilar artery embolism was induced by 40% FeCl3 solution. Changes of cochlear blood flow (CBF) before and after embolization were measured by laser Doppler flowmeter. And pre-amplifier devices such as synchronous monitoring of mean arterial pressure (MABP) changes. Results: The CBF started to decrease after 4 ~ 10% FeCl3 solution was induced for 5 ~ 10min, and the CBF decreased further with the prolongation of induction time. The decrease of CBF reached (4.5 ± 4.2)% at 50 min, The difference was significant (P <0.01). The mean CBF value was (81.3 ± 12.0)% throughout the experimental period, with a significant difference (P <0.01) as compared with that before induction. MABP no significant change. CONCLUSION: The application of 40% FeCl3 solution can induce basilar artery embolism in guinea pigs and provide an ideal animal model for studying the mechanism of microcirculation disturbance in the inner ear caused by arterial embolism and the development and application of antithrombotic drugs