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以提纯的肠炎沙门氏菌染色体ＤＮＡ为材料，在一对含有ＣＵＡＣＵＡＣＵＡＣＵＡ的特殊引物引导下，应用ＰＣＲ扩增出鞭毛蛋白基因ｆｌｉＣｇ，ｍ，约１．８ｋｂ左右大小，然后以ＵＤＧ法快速克隆到质粒ｐＡＭＰ１０中，转化第１宿主大肠杆菌ＴＧ１或大肠杆菌ＤＨ５α，在氨苄青霉素平板上筛选转化子，小量制备重组质粒ＤＮＡ，再转入第２宿主大肠杆菌ＬＣ－２ａ（ｈａｇ－，ｒｅｃＡ－），所有转化子均出现动力。其中的一个克隆ｐＡＭＰ·ＧＭ经动力和动力抑制试验、血清学试验、鞭毛电镜观察及部分序列测定，均证实ｐＡＭＰ·ＧＭ中载有肠炎沙门氏菌鞭毛蛋白基因ｆｌｉＣｇ，ｍ。ｆｉｌＣｇ，ｍ克隆成功为寻求肠炎沙门氏菌防制新方法奠定了基础，研究中建立的快速克隆策略为广泛、深入地开展ｆｌｉＣ研究提供了便捷的新途径。
The purified genomic DNA of Salmonella enteritidis was used as a material to amplify the flagellin gene fliCg, m, about 1.8 kb under the guidance of a pair of special primers containing CUACUACUACUA, and then quickly cloned into the plasmid pAMP10 by the UDG method , Transformed into the first host E. coli TG1 or E. coli DH5α, the transformants were screened on ampicillin plates, a small amount of recombinant plasmid DNA was prepared and transformed into the second host E. coli LC-2a (hag-, recA-) Children are motivated. One of the clones, pAMP GM, was tested for motility and kinetics, serological test, flagella electron microscopy and partial sequence analysis, confirming the presence of flagellar protein fliCg, m. The successful cloning of filCg and mCase laid the foundation for the search for a new control method of Salmonella enteritidis. The rapid cloning strategy established in this study provided a new and convenient way for the extensive and in-depth study of fliC.