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目的建立可用于椎间盘缺损修复研究的纤维环部分缺损动物模型。方法使用image J 1.46r软件测量5根羊脊柱X线片T_12/L1~L6/S1椎间隙高度,轴向剖开T_12/L1~L6/S1椎间盘,测量拟作缺损处纤维环厚度,使用11号尖刀在3根离体羊脊柱T_12/L1~L5/6椎间盘左前方制作上底3 mm、下底5 mm、高5 mm、厚3 mm,下底朝向髓核的梯形缺损,上述羊脊柱均取自1岁龄绵羊。采用微创外侧手术入路在2只1岁龄绵羊T_12/L1~L5/6椎间盘左前方制作相同规格的梯形缺损。通过轴向剖开椎间盘测量梯形缺损的边长、对切取的纤维环和少量髓核称质量,以此评估所制作的椎间盘梯形缺损。结果绵羊腰椎间隙高度为(4.45±0.28)mm,拟作缺损处纤维环厚度为(4.08±0.50)mm,分别与拟作梯形缺损的厚度(3 mm)和高度(5 mm)差异有统计学意义(P<0.05);离体羊腰椎间盘梯形缺损的上底(3.03±0.09)mm、下底(5.03±0.09)mm、高度(4.97±0.10)mm和厚度(3.02±0.06)mm分别与梯形缺损预定值(上底3mm、下底5 mm、高度5 mm和厚度3 mm)之间的差异无统计学意义(P>0.05);从离体和在体的羊腰椎间盘梯形缺损处取出的纤维环及髓核质量分别为(0.162±0.011)g和(0.166±0.014)g,两者差异无统计学意义(P>0.05)。结论通过微创外侧手术入路,使用实验中制作梯形缺损的方法可以简单、安全、可靠地制作出符合要求、能用于椎间盘缺损修复研究的羊纤维环部分缺损的动物模型。
OBJECTIVE: To establish an animal model of partial annulus fibrosus for the study of disc defect repair. Methods The height of intervertebral space of 5 sheep spine X-ray films T_12 / L1 ~ L6 / S1 was measured by image J 1.46r software and the thickness of the fibril ring was measured by T 11 / L 6 / The sharp knives were made on the front left of the intervertebral disc of T_12 / L1 ~ L5 / 6 of 3 isolated sheep spine with 3 mm in the upper, 5 mm in the lower, 5 mm in height, 3 mm in thickness, trapezoid defect toward the nucleus pulposus, 1 year old sheep. Minimally invasive lateral approach was used to make trapezoidal defect of the same size in front of T_12 / L1 ~ L5 / 6 intervertebral disc of two 1-year-old sheep. By traversing the intervertebral disc to measure the length of the trapezoid defect, the quality of the annulotomy and a small amount of the nucleus pulposus was weighed to evaluate the trapezoidal defect of the intervertebral disc. Results The lumbar intervertebral height was (4.45 ± 0.28) mm in sheep and (4.08 ± 0.50) mm in the intended defect. There was significant difference between the trabecular defect thickness (3 mm) and the height (5 mm) (3.03 ± 0.09) mm, lower base (5.03 ± 0.09) mm, height (4.97 ± 0.10) mm and thickness (3.02 ± 0.06) mm, respectively, were significantly associated with trapezoidal defects There was no significant difference between the pre-determined values (3 mm in the top, 5 mm in the bottom, 5 mm in height and 3 mm in thickness) (P> 0.05) The quality of ring and nucleus pulposus was (0.162 ± 0.011) g and (0.166 ± 0.014) g, respectively, with no significant difference (P> 0.05). Conclusion By using the minimally invasive lateral approach, the method of making trapezoidal defect in the experiment can be used to make a simple, safe and reliable animal model of partial defect of sheep fibrous ring which can meet the requirements and can be used in disc defect repair.