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为提高栽野稻杂种后代花药培养效率,几年来用不同的处理方法接种302个株系360660个花药,平均愈伤组织诱导率5.58%,最高诱导率33.33%,平均绿苗分化率25.11%,最高绿苗分化率91.67%,突破栽野稻杂交后代F1、F2低世代绿苗分化率低的难关,获得一批带野生稻优异种质的花药绿苗与稳定品系。
In order to improve the anther culture efficiency of offspring hybrid rice in the past few years, 360660 anthers of 302 lines were inoculated with different treatments, the average callus induction rate was 5.58% and the highest induction rate was 33.33% Rate of 25.11%, the highest differentiation rate of 91.67% green seedlings, crosses offspring F1 hybrid F1 generation and F2 low generational green seedling differentiation rate is low, access to a number of wild rice with excellent germplasm of anther green seedlings and stability strain.