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目的:探讨香加皮杠柳苷(cortex periplocae,CPP)联用肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor related apoptosis inducing ligand,TRAIL)对胃癌细胞的作用及其机制。方法:人胃癌细胞系SGC-7901、MGC-803培养完成后,采用50、100、200 ng/ml的CPP和1μg/ml的TRAIL单用或联合处理。MTS法检测SGC-7901和MGC-803细胞的增殖情况,流式细胞术检测其凋亡率,Western boltting检测pro-BID、Mcl-1、cleaved caspase-3、DR4、DR5的表达水平。结果:SGC-7901和MGC-803细胞经CPP(50、100、200 ng/ml)和TRAIL(1μg/ml)联合处理24 h后,细胞增殖率明显低于空白对照组和对应的CPP各剂量单独处理组(P<0.05或P<0.01)。SGC-7901和MGC-803细胞凋亡率均明显高于空白对照组和对应的CPP各剂量单独处理组(P<0.05或P<0.01)。SGC-7901和MGC-803细胞中pro-BID、Mcl-1表达水平明显降低(均P<0.05),cleaved caspase-3表达水平明显升高(P<0.05),DR4和DR5受体的表达水平升高(均P<0.05)。结论:CPP协同TRAIL可明显诱导人胃癌SGC-7901和MGC-803细胞凋亡,增强人胃癌细胞对TRAIL的敏感性。
AIM: To investigate the effect and mechanism of tumor necrosis factor related apoptosis inducing ligand (TRAIL) on gastric cancer cells by cortex periplocae (CPP). Methods: Human gastric cancer cell lines SGC-7901 and MGC-803 were treated with 50,100,200 ng / ml CPP and 1μg / ml TRAIL alone or in combination. The proliferation of SGC-7901 and MGC-803 cells was detected by MTS method. The apoptosis rate of SGC-7901 and MGC-803 cells was detected by flow cytometry. The expression of pro-BID, Mcl-1, cleaved caspase-3, DR4 and DR5 were detected by Western blotting. Results: The proliferation rates of SGC-7901 and MGC-803 cells treated with CPP (50,100,200 ng / ml) and TRAIL (1μg / ml) for 24 h were significantly lower than those of the blank control group and the corresponding CPP Groups were treated alone (P <0.05 or P <0.01). The apoptotic rates of SGC-7901 and MGC-803 cells were significantly higher than those of blank control group and corresponding CPP groups (P <0.05 or P <0.01). The expression of pro-BID and Mcl-1 in SGC-7901 and MGC-803 cells were significantly decreased (all P <0.05), and the expression of cleaved caspase-3 was significantly increased (P <0.05) (All P <0.05). CONCLUSION: CPP combined with TRAIL can obviously induce the apoptosis of human gastric cancer SGC-7901 and MGC-803 cells and enhance the sensitivity of human gastric cancer cells to TRAIL.