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目的观察心房颤动患者心房组织Ⅲ类组蛋白去乙酰化酶1(SIRT1)的表达及其与心房纤维化的关系。方法选择38例行心脏手术的风湿性心脏病患者,按照是否合并房颤分为两组:持续性房颤组25例(AF组),窦性心律组13例(SR组),于心脏手术时切取小块右心耳组织,采用实时荧光定量PCR法检测SIRT1mRNA的表达量,采用免疫组化法检测SIRT1蛋白表达量,行心脏超声评价心房结构情况,采用masson染色评价心房纤维化程度,比较两组患者SIRT1表达及评价SIRT1表达与心房纤维化的关系。结果 AF组患者SIRT1mRNA表达量高于SR组(P<0.05),AF组SIRT1蛋白表达量高于SR组(P<0.05),AF组SIRT1mRNA及蛋白表达量与心房内径无相关性(左房:r=0.198,r=0.047;右房:r=0.027,r=0.096,P均>0.05),AF组SIRT1mRNA及蛋白表达量与心房胶原容积分数呈负相关(r=-0.643,r=-0.721,P<0.05)。结论房颤患者心房组织SIRT1表达增加,与心房纤维化呈负相关,提示SIRT1可通过抑制心房纤维化在房颤中起作用。
Objective To investigate the expression of atrial histone deacetylase 1 (SIRT1) in atrial fibrillation and its relationship with atrial fibrosis. Methods Thirty-eight patients with rheumatic heart disease undergoing cardiac surgery were divided into two groups according to whether they were complicated with atrial fibrillation: 25 patients with AF (AF group), 13 patients with sinus rhythm (SR group) The right atrial appendage was cut out, the expression of SIRT1 mRNA was detected by real-time fluorescence quantitative PCR, the expression of SIRT1 protein was detected by immunohistochemistry, the atrial structure was evaluated by echocardiography, and the degree of atrial fibrosis was evaluated by masson staining. SIRT1 expression in patients and the relationship between SIRT1 expression and atrial fibrosis. Results The expression of SIRT1 mRNA in AF patients was significantly higher than that in SR patients (P <0.05). The expression of SIRT1 protein in AF patients was significantly higher than that in SR patients (P <0.05). There was no correlation between SIRT1 mRNA and protein expression in AF patients (left atrium: r = 0.198, r = 0.047; right atrium: r = 0.027, r = 0.096, all P> 0.05). The expression of SIRT1 mRNA and protein in AF group was negatively correlated with the volume fraction of atrial collagen (r = -0.643, r = , P <0.05). Conclusions SIRT1 expression is increased in atrial fibrillation patients with atrial fibrillation, which is negatively correlated with atrial fibrosis, suggesting that SIRT1 may play a role in atrial fibrillation by inhibiting atrial fibrosis.