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通过105 d的恒温(25℃)控湿室内培养方法,探讨了华北平原高产粮田土壤有机碳矿化特征以及水分和有机、无机氮输入对其影响。试验设4个肥料添加水平和4个水分梯度,分别为对照(S0)、仅添加无机氮(尿素)(S1)、无机氮和有机氮(鸡粪)配施(S2)以及仅添加有机氮(S3)和25%(田间持水量;M0)、50%(M1)、75%(M2)和100%(M3)共16个处理,每处理3次重复。结果表明,各处理有机碳矿化速率均在培养后1 d达第1高峰,之后直线下降,培养7 d时下降幅度达57.2%—75.0%,培养20—30 d时出现第2高峰。有机碳累积矿化量有208.8—1161 mg/kg,主要集中在前30 d,可占整个培养期的59.1%—69.9%,105 d的净矿化率为0.07%—2.01%。根据双指数方程模拟结果,研究了土壤潜在矿化碳库(C1+C2),其中活性碳库(C1)和惰性碳库(C2)分别为53.0—135.1 mg/kg和156.9—1069 mg/kg,潜在矿化率为1.75%—9.66%。土壤含水量显著影响有机碳矿化,且与潜在矿化碳库呈二次函数关系(P<0.05)。田间持水量25%—100%范围内,随着土壤含水量的升高,有机碳矿化速率呈增加趋势,但增幅降低,其中M2(田间持水量75%)的有机碳净矿化率最高。有机碳矿化量与土壤微生物碳和矿质氮含量呈线性正相关(P<0.05),保持氮水平(200 kg N/hm2)相同,有机氮(鸡粪)和无机氮(尿素)均显著促进土壤有机碳矿化,但两者间差异不显著(P>0.05),且有机氮和无机氮对有机碳矿化的影响均与土壤含水量有显著交互作用(P<0.05)。
Through 105 days of constant temperature (25 ℃) controlled indoor cultivation method, the effects of soil organic carbon mineralization and water and organic and inorganic nitrogen inputs on the grain yield of North China Plain were discussed. Four fertilizer addition levels and four water gradients were set up as control (S0), inorganic nitrogen (S1) only, inorganic nitrogen and organic nitrogen (chicken manure) (S2), and organic nitrogen (S3) and 25% (field capacity; M0), 50% (M1), 75% (M2) and 100% (M3) for a total of 16 treatments with 3 replicates per treatment. The results showed that the mineralization rates of organic carbon in all treatments reached the first peak on the first day after culture and then decreased linearly. The decline rate reached 57.2% -75.0% on the seventh day and reached the second peak on the 20th day. The cumulative mineralization of organic carbon was 208.8-1161 mg / kg, which mainly concentrated in the first 30 days, accounting for 59.1% -69.9% of the whole cultivation period. The net mineralization rate of 105 days was 0.07% -2.01%. According to the simulation results of double exponential equation, the soil potential mineralized carbon pool (C1 + C2) was studied, in which the activated carbon pool (C1) and the inert carbon pool (C2) were 53.0-135.1 mg / kg and 156.9-1069 mg / kg , The potential mineralization rate of 1.75% -9.66%. Soil moisture significantly affected the mineralization of organic carbon, and showed a quadratic functional relationship with the potential mineralized carbon pool (P <0.05). Within the range of 25% -100% of the field water holding capacity, the rate of organic carbon mineralization increased with the increase of soil water content, but the rate of increase decreased. Among them, M2 (field water holding capacity of 75%) had the highest rate of organic carbon net mineralization . Organic carbon mineralization had a linear positive correlation with soil microbial biomass carbon and mineral nitrogen (P <0.05), with same level of nitrogen (200 kg N / hm2), organic nitrogen (chicken manure) and inorganic nitrogen Soil organic carbon mineralization, but the difference between the two was not significant (P> 0.05), and organic nitrogen and inorganic nitrogen on organic carbon mineralization had a significant interaction with soil moisture (P <0.05).