【摘 要】
：
目的观察亚硝基吡啶在细胞恶性转化过程中的促癌作用.方法用HPV18E6E7诱导食管上皮细胞永生化细胞系SHEE,第17代细胞培养在50 ml培养瓶.加入亚硝基吡啶(Nnitrosopiperidine,N
【机 构】
：
汕头,中国疾病预防控制中心病毒病预防控制研究所
【基金项目】
：
国家高技术研究发展计划(863计划);
论文部分内容阅读
目的观察亚硝基吡啶在细胞恶性转化过程中的促癌作用.方法用HPV18E6E7诱导食管上皮细胞永生化细胞系SHEE,第17代细胞培养在50 ml培养瓶.加入亚硝基吡啶(Nnitrosopiperidine,NPIP)0,2,4,8 mmol/L作用3周.用相差显微镜检查细胞形态,流式细胞仪检测细胞增殖和凋亡;染色体常规制样,检查染色体众数;细胞软琼脂集落形成及接种裸小鼠检查成瘤性;用Western blot检测HPV18表达.结果当细胞暴露在8 mmol/L NPIP时细胞死亡增加,只剩少量活细胞.换正常培基代替NPIP,经4周后细胞进入增殖状态,细胞出现增生和异型增生.第8周末细胞软琼脂培养有大集落形成,接种裸小鼠成瘤.2,4 mmol/L组细胞倍增时间延长,细胞未能成瘤.8 mmol/L NPIP组染色体众数61～65,对照组56～61.实验组和对照组HPV阳性.结论NPIP促进人乳头状瘤病毒诱导人胚食管永生化上皮恶性转化,HPV18E6E7和NPIP能协同作用加速食管上皮恶性转化.
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