论文部分内容阅读
目的研究吴茱萸碱(Evo)对人胃腺癌(SGC-7901)细胞的作用,并初步探讨其作用机制。方法 MTT法测定Evo的抗肿瘤活性;电镜观察Evo对细胞形态学的影响;琼脂糖凝胶电泳检测Evo对细胞DNA电泳行为的影响;流式细胞技术检测Evo对SGC-7901细胞的抑制作用和细胞周期的影响。结果一定浓度范围内Evo对SGC-7901细胞的增殖有剂量、时间依赖性抑制作用;透射电镜下可见细胞核染色质边集、核碎裂及凋亡小体;一定浓度的Evo可使细胞基因组DNA电泳出现阶梯状条带;流式细胞仪检测Evo作用后,G0/G1期和S期细胞减少,大量细胞停滞在G2/M期,凋亡细胞增多。结论 Evo对SGC-7901细胞的抑制作用与诱导其凋亡使细胞停滞于G2/M期密切相关。
Objective To study the effect of evodiamine (Evo) on human gastric adenocarcinoma (SGC-7901) cells and to explore its mechanism. Methods The antitumor activity of Evo was assayed by MTT assay. The morphological changes of Evo were observed by electron microscopy. The effect of Evo on DNA electrophoresis was detected by agarose gel electrophoresis. The inhibitory effect of Evo on SGC-7901 cells was detected by flow cytometry. Effect of cell cycle. Results Evo could inhibit the proliferation of SGC-7901 cells in a dose-and time-dependent manner in a certain concentration range. The chromatin margins, nuclear fragmentation and apoptotic bodies were observed by transmission electron microscopy. Evo at a certain concentration could induce genomic DNA The ladder-like bands appeared after electrophoresis. After Evo was detected by flow cytometry, the number of cells in G0 / G1 phase and S phase decreased, a large number of cells were arrested in G2 / M phase and apoptotic cells increased. Conclusion The inhibitory effect of Evo on SGC-7901 cells is closely related to the induction of apoptosis by cell cycle arrest in G2 / M phase.