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脯氨酸在植物渗透调节中起举足轻重的作用,1-吡咯啉-5-羧酸合成酶(P5CS)是脯氨酸的谷氨酸合成途径中的关键酶,为了研究玉米P5CS基因家族与玉米抗逆性的关系,对玉米幼苗进行了非生物胁迫(干旱、低温、盐胁迫)处理,利用半定量RT-PCR技术分析了ZMP5CS基因家族在不同胁迫条件下的表达特性。结果表明,ZMP5CS基因家族中除ZM02G27230以外的基因不论地上部叶还是地下部根均被干旱胁迫诱导上调表达,但各成员之间其表达量最高点出现的时间不同;相同处理同一基因相同的处理时间点地上部与地下部基因表达量存在差异,在干旱胁迫下ZM08G14210的表达量最高,在盐胁迫下ZM08G31890的表达量最高,在低温胁迫下ZM06G25580的表达量最高,说明ZMP5CS基因家族表达受非生物胁迫的诱导。
Proline plays a pivotal role in plant osmotic regulation. 1-Pyrroline-5-carboxylate synthase (P5CS) is a key enzyme in the glutamate synthesis pathway of proline. In order to study the relationship between P5CS gene family and maize Stress tolerance of maize seedlings. Abiotic stress (drought, low temperature, salt stress) was applied to the maize seedlings. Semi-quantitative RT-PCR was used to analyze the expression of ZMP5CS gene family under different stress conditions. The results showed that the genes except ZM02G27230 in ZMP5CS gene family were up-regulated by drought stress, but the highest expression point appeared at different time. The same treatment with the same gene The expression of ZM08G14210 was the highest under drought stress, the highest expression of ZM08G31890 under salt stress and the highest expression of ZM06G25580 under low temperature stress, which indicated that the expression of ZMP5CS gene family was affected by non-specific Induction of biotic stress.