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目的 克隆并鉴定Lin-CD3 4-细胞区别于Lin-CD3 4+细胞的可能与其性状相关的基因。方法 以Lin-CD3 4-细胞作为检测对象 ,Lin-CD3 4+细胞作为驱动细胞 ,应用抑制消减杂交技术构建Lin-CD3 4-细胞的cDNA消减文库。选取部分克隆测序 ,测序结果与GenBank进行同源性比较和功能分析。结果 共获得 593个含有插入片段的克隆 ,片段的长度为 30 0～ 50 0bp。随机选取 53条EST进行测序 ,其中 37条EST代表了 1 0个已知基因 ,其余 1 6条EST代表了 4个未知序列。结论 利用抑制消减杂交技术鉴定出部分Lin-CD3 4-细胞特异表达的基因 ,可能与Lin-CD3 4-细胞的特性相关
Objective To clone and identify genes that may be related to traits of Lin-CD3 4-cells as distinct from Lin-CD3 4+ cells. Methods Lin-CD3 4-cells were used as the detection target and Lin-CD3 4+ cells as the driving cells. The cDNA subtractive library of Lin-CD3 4-cells was constructed by suppression subtractive hybridization. Selected partial cloning and sequencing, sequencing results and GenBank homology comparison and functional analysis. As a result, a total of 593 clones containing the inserted fragment were obtained. The length of the fragment was 30 0 ~ 50 0bp. A total of 53 ESTs were randomly selected for sequencing. 37 ESTs represented 10 known genes and the remaining 16 ESTs represented 4 unknown sequences. Conclusion Identification of some genes specifically expressed in Lin-CD3 4-cells by suppression subtractive hybridization may be related to the characteristics of Lin-CD3 4-cells