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目的研究Ⅲ、Ⅳ型胶原在ＭｓＰＧＮ中的变化情况，同时探讨图像分析仪在此病中的诊断意义。方法将临床确诊为系膜增生性肾小球肾炎（ＭｓＰＧＮ）的病例分为轻、中、重三组，从每组中随机抽取１０例作为实验组１、２、３，取３例正常肾组织作为对照组。然后对所选取的３３例分别进行光镜图像分析，间接免疫荧光检查。结果在Ｍａｓｏｎ染色的绿染部分分析结果中，中、重度ＭｓＰＧＮ与正常比较有显著性差异（Ｐ＜０．０５），ＰＡＳＭ黑染中，重度与正常比较有显著性差异（Ｐ＜０．０５）。间接免疫荧光见：对照组肾小球内未见Ⅲ型胶原，有少量Ⅳ型胶原分布于毛细血管基膜及系膜区，Ⅲ型胶原在中，重度ＭｓＰＧＮ中出现，Ⅳ型胶原随着病变进展明显增加。结论Ⅲ、Ⅳ型胶原的出现及增加在肾小球肾炎的病变过程中起着重要作用。图像分析技术对肾小球肾炎的诊断具有一定的临床价值。
Objective To study the changes of type Ⅲ and type Ⅳ collagen in MsPGN and to discuss the diagnostic significance of image analyzer in this disease. Methods The cases diagnosed as mesangial proliferative glomerulonephritis (MsPGN) were divided into three groups: light, medium and heavy. Ten cases were randomly selected from each group as experimental group 1, Tissue as a control group. Then 33 cases were selected for light microscopic image analysis, indirect immunofluorescence. Results Among the results of Mason stained green staining, the moderate and severe MsPGN were significantly different from the normal ones (P <0.05). There was a significant difference between moderate and severe black stains in PASM (P <0.05) ). Indirect immunofluorescence showed that there was no type III collagen in the glomeruli in the control group, a small amount of type IV collagen distributed in capillary basement membrane and mesangial area, type III collagen appeared in medium and severe MsPGN, The progress has obviously increased. Conclusion The appearance and increase of type Ⅲ and type Ⅳ collagen plays an important role in the pathological changes of glomerulonephritis. Image analysis technology for the diagnosis of glomerulonephritis has some clinical value.