论文部分内容阅读
目的:探讨再生基因Reg IV在多囊卵巢综合征(PCOS)模型大鼠卵巢组织中的表达情况。方法:采用Poretsky法用胰岛素联合hCG诱导SD大鼠建立PCOS模型,观察大鼠体质量变化及卵巢相对质量;测定血清T、LH、FSH、E2、Ins水平;HE染色观察卵巢形态改变;免疫组织化学方法检测Reg IV蛋白在卵巢组织的定位表达;定量RT-PCR和Western blotting分别检测Reg IV在mRNA水平和蛋白水平的表达。结果:Reg IV分别表达于大鼠颗粒细胞、卵泡膜细胞以及黄体等部位;模型组卵巢中Reg IV mRNA及蛋白表达均低于正常对照组(P<0.05)。结论:Reg IV的表达下调可能与PCOS的发病有关。
Objective: To investigate the expression of Reg IV gene in ovarian tissue of rats with polycystic ovary syndrome (PCOS). Methods: PCOS model was induced by Poretsky’s method with insulin combined with hCG in rats. The changes of body weight and the relative quality of ovaries were observed. The levels of serum T, LH, FSH, E2 and Ins were measured. The morphological changes of ovary were observed by HE staining. The expression of Reg IV protein in ovarian tissue was detected by chemical methods. The expression of Reg IV at mRNA and protein levels was detected by quantitative RT-PCR and Western blotting respectively. Results: Reg IV was expressed in granulosa cells, theca cells and corpus luteum in rats, respectively. Reg IV mRNA and protein expression in ovary of model group were lower than those in normal control group (P <0.05). Conclusion: The down regulation of Reg IV may be related to the pathogenesis of PCOS.