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目的:研究新疆家蚕抗菌肽(cecropin-XJ,CE-XJ)与4种化疗药物联用对人食管癌Eca109细胞增殖及凋亡的影响。方法:MTT法检测CE-XJ联合不同浓度的多柔比星(doxorubicin,ADM)、卡铂(carboplatin,CBP)、顺铂(cisplatin,DDP)和环磷酰胺(cyclophosphamide,CTX)对人食管癌Eca109细胞增殖的影响,流式细胞术分析CE-XJ与ADM、CBP、DDP、CTX联用对Eca109细胞凋亡、活性氧及线粒体膜电位的影响。结果:单独使用CE-XJ(1~50μmol/L)可依剂量依赖方式显著抑制Eca109细胞的增殖(P<0.05或P<0.01)。CE-XJ分别与ADM、DDP、CTX联合用药,与单独用药相比,Eca109细胞的增殖抑制明显加强、细胞凋亡率显著增加(均P<0.05或P<0.01),细胞内活性氧水平显著增加(P<0.05),而线粒体膜电位显著降低(均P<0.05)。但CE-XJ与CBP联用时出现拮抗现象,与单独用药相比,细胞增殖抑制变化不大,细胞凋亡率反而降低。结论:CE-XJ与化疗药物ADM、DDP和CTX联用对人食管癌Eca109细胞的增殖抑制和凋亡诱导有明显促进作用,其机制可能与细胞内活性氧和线粒体膜电位变化有关。但CE-XJ与CBP联用则起拮抗作用,其机制有待进一步探讨。
Objective: To study the effects of cecropin-XJ (Cecropin-XJ) and four chemotherapeutic agents on the proliferation and apoptosis of human esophageal carcinoma Eca109 cells. Methods: The effect of CE-XJ combined with different concentrations of doxorubicin (ADM), carboplatin (CBP), cisplatin (DDP) and cyclophosphamide (CTX) Eca109cells proliferation, flow cytometry analysis of CE-XJ and ADM, CBP, DDP, CTX combination of Eca109cells apoptosis, reactive oxygen species and mitochondrial membrane potential. Results: CE-XJ alone (1 ~ 50μmol / L) significantly inhibited the proliferation of Eca109 cells in a dose-dependent manner (P <0.05 or P <0.01). CE-XJ combined with ADM, DDP and CTX, respectively, inhibited the proliferation of Eca109 cells and significantly increased the apoptosis rate (all P <0.05 or P <0.01) (P <0.05), while mitochondrial membrane potential decreased significantly (all P <0.05). However, CE-XJ combined with CBP appeared antagonistic phenomenon, compared with the drug alone, little change in cell proliferation inhibition, but the rate of apoptosis decreased. Conclusions: The combination of CE-XJ and chemotherapy drugs ADM, DDP and CTX can significantly promote the proliferation and apoptosis of human esophageal cancer Eca109 cells. The mechanism may be related to changes of intracellular reactive oxygen species and mitochondrial membrane potential. However, CE-XJ and CBP play an antagonistic role, and its mechanism needs to be further explored.