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目的:观察虎纹捕鸟蛛毒素(HWTX-I)对全脑缺血再灌注大鼠海马CA1区锥体细胞Nissl染色形态学变化、脑组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力、丙二醛(MDA)浓度变化的影响,探讨其改善全脑缺血再灌注损伤的机制,为颅脑损伤的临床治疗提供新的有效治疗手段。方法:SD大鼠随机分为假手术组、生理盐水组及三组用药组,采用改良的Pulsinelli四血管阻断全脑缺血损伤模型结合蛛网膜下腔置管术,对大鼠海马锥体细胞进行Nissl染色及测定各组大鼠脑组织SOD、CAT活力及MDA浓度。结果:1)HWTX-I能明显减轻全脑缺血大鼠海马锥体细胞的损伤。2)HWTX-I能使全脑缺血再灌注大鼠脑组织SOD、CAT活力明显升高,MDA浓度明显降低。结论:HWTX-I对全脑缺血再灌注等引起的大鼠大脑神经细胞损伤有明显的保护作用。
Objective: To observe the change of Nissl staining of pyramidal neurons in hippocampal CA1 area of cerebral ischemic reperfusion rats treated with HWTX-I, the changes of superoxide dismutase (SOD), catalase (CAT) activity and malondialdehyde (MDA) concentration in rats, and to explore its mechanism of improving global cerebral ischemia-reperfusion injury and to provide a new and effective treatment for the treatment of traumatic brain injury. Methods: SD rats were randomly divided into sham-operated group, saline group and three groups. The rats were subjected to four-vessel occlusion with modified Pulsinelli global cerebral ischemia model combined with subarachnoid catheterization, Cell Nissl staining and determination of the rat brain tissue SOD, CAT activity and MDA concentration. Results: 1) HWTX-I can significantly reduce the damage of hippocampal pyramidal cells in rats with global cerebral ischemia. 2) HWTX-I can significantly increase the activity of SOD and CAT and decrease the concentration of MDA in the brain of rats with global cerebral ischemia / reperfusion. Conclusion: HWTX-I can protect rat’s brain from neuronal injury caused by global cerebral ischemia and reperfusion.