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采用砂基栽培,研究不同铝胁迫强度(0、50、100、150、200μmol.L-1)和胁迫时间(30d、60d和100d)条件下,对马尾松(Pinus massoniana)幼苗单株根长、根干重、根系质膜透性、根系吸收总面积、活跃吸收面积和根系活力的影响。结果表明:低水平胁迫(0~50μmol.L-1)对马尾松幼苗根系生理指标影响较小;中等水平胁迫(100μmol.L-1)是马尾松根系生长和活力变化的转折点;重度胁迫(150~200μmol.L-1)条件下,根系生理指标呈现大幅度下降变化趋势。根长、根质量、质膜透性和根系活力可作为马尾松幼苗根系受铝毒害的鉴定指标。短时间(<30d)根际供铝,马尾松幼苗适应能力较强,根系生长抑制不明显;栽培30d以上时,马尾松根部对铝胁迫的响应随胁迫时间的增加而加剧。
Sand-based cultivation was used to study the effects of different aluminum stress intensity (0, 50, 100, 150 and 200 μmol.L-1) and stress time (30 d, 60 d and 100 d) on Pinus massoniana seedling root length Root dry weight, permeability of the plasma membrane of the root system, total root absorption area, active absorption area and root activity. The results showed that low level stress (0-50 μmol·L-1) had little effect on the physiological index of Pinus massoniana seedling roots. Medium level stress (100 μmol·L-1) was the turning point of root growth and vigor of masson pine. Under the condition of 150 ~ 200μmol.L-1, the physiological index of root showed a decreasing trend. Root length, root quality, plasma membrane permeability and root activity could be used as the identification index of P. massoniana seedlings root toxicity by aluminum. Short time (<30 days), the response to aluminum stress in Pinus massoniana seedlings increased with the increase of stress time, but the growth of Pinus massoniana seedlings was not obvious.