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目的:探讨CD4+CD25+CD127-调节性T细胞在大肠癌患者外周血中的表达水平及临床意义。方法:应用流式细胞仪检测200例大肠癌患者外周血CD4+CD25+CD127-调节性T细胞占CD4+T细胞的百分比,并分析其与大肠癌组织的分化程度、淋巴结转移和临床分期的关系。结果:大肠癌患者外周血CD4+CD25+CD127-调节性T细胞占CD4+T细胞的百分率〔(4.84±1.35)%〕明显高于健康对照组〔(0.85±0.25)%〕,差异有统计学意义,P<0.05。低分化者外周血调节性T细胞〔(4.21±0.42)%〕明显高于高分化者〔(3.92±0.41)%〕,差异有统计学意义,P<0.05;有淋巴结转移者外周血调节T细胞〔(4.57±1.44)%〕明显高于无淋巴结转移者〔(2.36±0.68)%〕,差异有统计学意义,P<0.01;Ⅲ和Ⅳ期患者外周血调节性T细胞〔(3.53±1.41)%和(4.38±1.32)%〕明显高于Ⅰ～Ⅱ期〔(1.90±0.86)%〕,差异有统计学意义,P<0.01。结论:大肠癌患者外周血CD4+CD25+CD127调节性T细胞占CD4+T细胞的百分率明显升高,可能在大肠癌的免疫耐受和免疫逃逸中发挥重要作用,检测其结果对于判断大肠癌的病程进展及预后有一定参考价值。
Objective: To investigate the expression of CD4 + CD25 + CD127-regulatory T cells in peripheral blood of patients with colorectal cancer and its clinical significance. Methods: The percentage of CD4 + CD25 + CD127-regulatory T cells in CD4 + T cells in peripheral blood of 200 patients with colorectal cancer was detected by flow cytometry. The differentiation degree, lymph node metastasis and clinical stage of colorectal cancer relationship. Results: The percentage of CD4 + CD25 + CD127-regulatory T cells in CD4 + T cells (4.84 ± 1.35%) in peripheral blood of patients with colorectal cancer was significantly higher than that in healthy controls [(0.85 ± 0.25)%] Significance, P <0.05. (4.21 ± 0.42)% in peripheral blood of poorly differentiated patients was significantly higher than that in well-differentiated patients (3.92 ± 0.41)%, P <0.05; T lymphocyte subsets regulated by peripheral blood (4.57 ± 1.44)%〕 were significantly higher than those without lymph node metastasis 〔(2.36 ± 0.68)%〕, the difference was statistically significant, P <0.01; Ⅲ and Ⅳ patients with peripheral blood T regulatory cells 〔(3.53 ± 1.41% and 4.38 ± 1.32%, respectively) were significantly higher than those in stage Ⅰ ~ Ⅱ 〔(1.90 ± 0.86)%〕, the difference was statistically significant (P <0.01). Conclusion: The percentage of CD4 + CD25 + CD127 regulatory T cells in CD4 + T cells in peripheral blood of patients with colorectal cancer is significantly increased, which may play an important role in the immune tolerance and immune escape of colorectal cancer. To test the results for the determination of colorectal cancer The course of progress and prognosis of a certain reference value.