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目的探讨核转录因子-κB(nuclear fastor kappa B,NF-κB)在免疫性肝损伤小鼠模型中的作用。方法通过尾静脉注射BCG(125 mg/kg)14 d制备免疫性肝损伤小鼠模型。采用免疫组织化学法检测各组小鼠肝组织中NF-κB的表达程度。结果免疫组化结果显示,模型组肝细胞的NF-κB p65平均细胞阳性数明显增高,差异具有统计学意义(P<0.05);PDTC干预组肝组织中NF-κB p65的表达显著低于模型组,差异有统计学意义(P<0.05),且PDTC各剂量干预组之间肝组织中NF-κB p65的表达差异无统计学意义(P>0.05)。结论 PDTC可显著抑制免疫性肝损伤小鼠肝组织中NF-κB的表达,明显改善免疫性肝损伤肝组织病理表现。提示可通过PDTC抑制NF-κB的活化,干预肝损伤过程。
Objective To investigate the role of nuclear factor kappa B (NF-κB) in immune-induced liver injury in mice. Methods A mouse model of autoimmune liver injury was established by injecting tail vein injection of BCG (125 mg / kg) for 14 days. Immunohistochemistry was used to detect the expression of NF-κB in liver tissue of each group. Results The results of immunohistochemistry showed that the positive cells of NF-κB p65 in hepatocytes of model group were significantly increased (P <0.05), and the expression of NF-κB p65 in hepatic tissue of PDTC intervention group was significantly lower than that of model (P <0.05). There was no significant difference in the expression of NF-κB p65 in liver tissues between PDTC groups (P> 0.05). Conclusion PDTC can significantly inhibit the expression of NF-κB in the liver of mice with autoimmune liver injury and significantly improve the pathological manifestation of liver in autoimmune liver injury. Tip PDTC can inhibit the activation of NF-κB, interfere with the process of liver injury.