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目的鉴定脑脊液寡克隆区带(oligoclonal band,OB)的存在,探讨其在中枢神经系统疾病中的临床应用。方法对脑脊液和血清标本同时进行琼脂糖凝胶电泳,使用酶标抗人免疫球蛋白G,采用免疫固定电泳技术后,用TTF3显色。鉴定是否存在寡克隆区带。结果 67例神经系统疾病患者,其中4例临床确诊为多发性硬化症(multiple sclerosis,MS),经电泳免疫固定酶标显色后,2例(50%)发现寡克隆蛋白阳性,其他为阴性。结论脑脊液免疫固定电泳是检测脑脊液中内源性合成免疫球蛋白的直观方法。OB的检查对于多发性硬化和神经系统炎症有一定的诊断价值。
Objective To identify the presence of oligoclonal band (OB) in cerebrospinal fluid and to explore its clinical application in central nervous system diseases. Methods Cerebrospinal fluid (CSF) and serum samples were subjected to agarose gel electrophoresis at the same time. Enzyme - labeled anti - human immunoglobulin G was used. After immunostaining, the cells were stained with TTF3. Identify the presence of oligoclonal bands. RESULTS: Sixty-seven patients with neurological diseases were diagnosed as multiple sclerosis (MS). After electrophoresis and immunoprecipitation enzyme-linked immunosorbent assay, oligoclonal protein was found in two cases (50%) and negative in others . Conclusion CSF immunoelectrophoresis is an intuitive method for the detection of endogenous synthetic immunoglobulins in cerebrospinal fluid. OB examination for multiple sclerosis and nervous system inflammation have a certain diagnostic value.