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植物甾醇广泛分布于各种食用植物中,主要以游离态甾醇、甾醇酯、甾醇糖苷和脂酰基甾醇糖苷形式存在,具有抗炎、抗菌、抗真菌、降低胆固醇、抗肿瘤等功效。本文综述了近些年关于植物甾醇色谱检测方法的研究进展。植物甾醇常用样品前处理手段有液液萃取、薄层色谱、高速逆流色谱和超临界萃取等,而植物甾醇检测方法主要采用气相色谱法和液相色谱法,其中气相色谱法检测甾醇的灵敏度较高,但液相色谱法具有柱温温和、不破坏样品的特性,适合检测热不稳定植物甾醇。气相色谱法或液相色谱法与质谱检测器联用技术在多组分植物甾醇的同时定量方面取得了一定进展。
Phytosterols are widely distributed in a variety of edible plants, mainly in the form of free sterols, sterol esters, steryl glycosides and fatty acyl steryl glycosides, which have anti-inflammatory, antibacterial, antifungal, lower cholesterol and anti-tumor effects. This review summarizes recent research progress on the detection of phytosterols. Phytosterols commonly used in sample preparation methods are liquid-liquid extraction, thin-layer chromatography, high-speed countercurrent chromatography and supercritical fluid extraction. However, phytosterols are mainly detected by gas chromatography and liquid chromatography. The sensitivity of gas chromatography for the determination of sterols High, but liquid chromatography has a gentle column temperature, does not destroy the characteristics of the sample, suitable for detection of heat-labile phytosterols. Some progress has been made in the simultaneous quantification of multicomponent phytosterols by gas chromatography or liquid chromatography coupled with mass spectrometry.