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A new triterpenoid saponin, together with a known saponin, was isolated from the flowers of Lonicera Japonica Thunb. Using chemical and spectroscopic methods, mainly 2D NMR technique, their structures were deduced to be 3-βD-glucopyranosyl-(1→4)-βD-glucopyranosyl-(1→3)-α-L-rhamnospyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin 28-O-βD-glucopyranosyl-(1→6)-βD-glucopyranoside and 3-α-L-rhamnospyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnospyranosyl- (1→2)-[βD-xylcopyranosyl--(1→6)]-βD-glucopyranoside, respectively.