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目的观察促红细胞生成素(erythropoietin,EPO)对异丙肾上腺素(isoproterenol,ISO)引起大鼠心肌损伤及心肌细胞凋亡相关基因表达的影响。方法将雄性Sprague-Dawley(SD)大鼠分为3组:对照组、ISO组和EPO治疗组,检测血肌酸激酶、肌酸激酶同工酶水平及心肌caspase-3、iNOS的蛋白表达变化。结果EPO治疗组心肌酶水平及caspase-3、iNOS的蛋白表达均较ISO组明显减低。结论促红细胞生成素可以显著减轻异丙肾上腺素引起的心肌损伤,抑制caspase- 3、iNOS蛋白表达。
Objective To investigate the effect of erythropoietin (EPO) on myocardial injury and expression of cardiomyocyte apoptosis-related genes in rats induced by isoproterenol (ISO). Methods Male Sprague-Dawley (SD) rats were divided into 3 groups: control group, ISO group and EPO treatment group. The changes of serum creatine kinase, creatine kinase isoenzyme and the protein expression of caspase-3 and iNOS in myocardium were detected . Results The levels of myocardial enzymes and the expressions of caspase-3 and iNOS in EPO group were significantly lower than those in ISO group. Conclusion Erythropoietin can significantly reduce isoproterenol-induced myocardial injury and inhibit caspase-3 and iNOS protein expression.