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目的建立应用PF6-2型双道原子荧光光度计测定生物材料中总汞的方法。方法样品经湿法或微波消解后,用原子荧光光度法测定其汞含量。结果检出限为0.11 ng/ml,在0~10 mg/ml内线性关系良好,相关系数r为0.999 7。与现行测定汞的方法相比,测定结果差异无统计学意义(P>0.05)。微波消解的精密度相对较好,加标回收率在91.0%~104.0%之间。结论原子荧光光度法分析速度快,检出限低,精密度和准确度好,适合生物材料中总汞的测定。
Objective To establish a method for the determination of total mercury in biological materials by PF6-2 double channel atomic fluorescence spectrophotometer. Methods After the sample was digested by wet or microwave, its content of mercury was measured by atomic fluorescence spectrometry. The detection limit was 0.11 ng / ml. The linearity was good at 0-10 mg / ml with a correlation coefficient of 0.999 7. Compared with the current method for the determination of mercury, the difference was not statistically significant (P> 0.05). The precision of microwave digestion is relatively good, the spiked recoveries ranged from 91.0% to 104.0%. Conclusion The atomic fluorescence spectrometry has the advantages of fast analysis, low detection limit, good precision and accuracy, and is suitable for the determination of total mercury in biological materials.