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为了解河北省石家庄市健康儿童中肠道病毒带毒情况及血清型构成,对2010~2012年石家庄市的3个县区1 223例0~6岁的健康儿童及406名监护人(健康成人)采集咽拭子和粪便标本进行核酸检测鉴定肠道病毒血清型,并进行分子流行病学分析,为肠道病毒感染相关疾病的防控和治疗提供基础资料。1 223名健康儿童的标本检测结果中,肠道病毒(Enterovirus,EV)阳性的283例,阳性率为23.14%(283/1 223)。其中,以非肠道病毒71型非柯萨奇病毒A组16型的EV(Other-Enterovirus,other-EVs)阳性为主,占EV阳性的62%(175/283),而引起手足口病常见的EV病原体肠道病毒71型(Enterovirus A71,EV71)和柯萨奇病毒A组16型(Coxsackievirus A16,CVA16)阳性率较低,分别占19%(55/283)和18%(51/283);将结果为other-EVs阳性的175名健康儿童的标本全部进行病毒分离鉴定,共分离到25株病毒,分离率为14.29%(25/175),其中柯萨奇病毒B组5型(Coxsackievirus B5,CVB5)分离率最高,为40%(10/25)、柯萨奇病毒B组13型(Coxsackievirus B3,CVB3)分离率为24%(6/25)、埃可病毒21型(Enteric cytopathic human orphan virus 21,ECHO21)分离率为16%(4/25)、其他EV株分离率为20%(5/25)。提示,应加强对CVB3和CVB5等other-EVs的监测。
In order to understand the enterovirus infection status and serotype composition of healthy children in Shijiazhuang City of Hebei Province, 1 223 healthy children aged 0-6 years and 406 guardians (healthy adults) in 3 counties of Shijiazhuang from 2010 to 2012 were enrolled in this study. Collect throat swabs and stool specimens for nucleic acid detection and identification of enterovirus serotypes, and molecular epidemiological analysis for the prevention and treatment of enterovirus infection-related diseases and provide the basic information. Of 223 healthy children, 283 were positive for enterovirus (EV) positive test, with a positive rate of 23.14% (283/1 223). Among them, the majority of EV-positive cases were EV-positive (62% (175/283)) of EV-positive cases of non-enterovirus 71 non-Coxsackievirus A The common positive rates of Enterovirus A71 (EV71) and Coxsackievirus A16 (CVA16) were 19% (55/283) and 18% (51%), respectively, 283) .A total of 175 healthy children whose results were positive for other-EVs were all isolated and identified. Twenty-five isolates were isolated and the isolation rate was 14.29% (25/175). Among them, Coxsackievirus B (Coxsackievirus B5, CVB5) had the highest isolation rate of 40% (10/25), Coxsackievirus B3 (CVB3) isolation rate was 24% (6/25), and echovirus 21 Enteric cytopathic human orphan virus 21, ECHO21) isolated rate of 16% (4/25), other EV strains isolated rate of 20% (5/25). Tip, should be strengthened CVB3 and CVB5 other-EVs monitoring.