论文部分内容阅读
目的综述猪胰岛细胞分离纯化的常用方法及最新研究进展。方法查阅近年来国内外关于猪胰岛细胞分离纯化的相关文献,进行总结分析。结果分离纯化的效果主要取决于供体的前期筛选、高质量胰腺的获取与有效保存,以及分离纯化方法的选择与改进。结论猪胰岛分离纯化操作的优化与标准化的建立,极大促进了异种胰岛移植研究的发展,有望解决目前移植供体短缺的问题。
OBJECTIVE: To summarize the commonly used methods and recent research progress of porcine islet cell isolation and purification. Methods In recent years, domestic and foreign literature on the isolation and purification of porcine islet cells related literature for a summary analysis. Results The results of isolation and purification mainly depend on the preliminary screening of donors, the acquisition and effective preservation of high quality pancreas, and the selection and improvement of isolation and purification methods. Conclusion The optimization and standardization of isolation and purification of porcine islets have greatly promoted the development of research on xenotransplantation of islets and are expected to solve the current shortage of donor transplants.