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目的:研究联用中药“松友饮”和干扰素α(IFN-α)对高转移人肝癌裸鼠原位模型肿瘤生长、转移和生存期的影响。方法:以人高转移MHCC97H细胞建立裸鼠原位移植瘤模型,48只成模裸鼠随机分成对照组、“松友饮”组、IFN-α组及“松友饮”+IFN-α组,肝脏接种肿瘤24h开始每天1次灌胃给药及皮下注射,连续5周。第35天每组随机处死6只裸鼠,计算肿瘤体积及转移情况;剩余成瘤裸鼠用于观察生存期。结果:“松友饮”组、IFN-α组及“松友饮”+IFN-α组肿瘤体积、肺转移结节数及生存期与对照组比较,均有显著差异(P<0.05)。结论:连续5周联用“松友饮”显著增强IFNα对HCC裸鼠模型肿瘤生长、转移的抑制作用并延长生存期,对HCC病人有潜在的应用价值。
Objective: To study the effects of combined Chinese herbal medicine “Songyouyin” and interferon-α (IFN-α) on tumor growth, metastasis and survival in situ in nude mice with highly metastatic human hepatocellular carcinoma. Methods: Nude mice transplantation tumor model was established by human high metastasis MHCC97H cells. Forty-eight nude mice were randomly divided into control group, “Fuyou Yin” group, IFN-α group and “Songyouyin” + IFN- α group, liver tumor inoculation 24 hours a day once a day oral administration and subcutaneous injection for 5 weeks. On the 35th day, 6 nude mice were randomly sacrificed and the tumor volume and metastasis were calculated. The remaining tumor-bearing nude mice were used to observe the survival time. Results: The volume of tumor, the number of lung metastasis and the survival time of the rats in “Song Youyin” group, IFN-α group and “Songyouyin” + IFN-α group were significantly different from those in control group (P <0.05 ). CONCLUSION: Continuous 5 weeks combined with “Songyouyin” significantly enhances the inhibitory effect of IFNα on tumor growth and metastasis in HCC nude mice model and prolongs the survival time, which has potential value in HCC patients.