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目的 研究反义癌基因与抑癌基因联合抑制肝癌细胞生长的效果。方法 利用基因重组方法构建携带 p16基因、p5 3基因的融合表达载体 (pcDNA16 5 3) ,利用基因合成仪体外合成N ras、c myc反义寡核苷酸 ,并将N ras、c myc反义寡核苷酸与pcDNA16 5 3融合表达载体转染到人肝癌细胞系SMMC772 1细胞中 ,观察肝癌细胞生长情况及软琼脂集落形成能力。结果 联合治疗组肝癌细胞增殖速度最慢 ,软琼脂集落形成个数最少 ,只有 2个。结论 利用相关基因联合治疗效果明显优于利用个别基因的治疗效果。
Objective To study the combined effect of antisense oncogene and tumor suppressor gene on the growth of hepatoma cells. Methods The fusion gene p16 gene and p5 3 gene (pcDNA16 5 3) was constructed by gene recombination method. Antisense oligonucleotides N ras and c myc were synthesized by gene synthesizer in vitro. Antisense N ras and c myc The oligonucleotide and pcDNA165 fusion expression vector was transfected into human hepatoma cell line SMMC772 1 cells to observe the growth of hepatoma cells and the ability of soft agar colony formation. Results The liver cancer cells in the combination therapy group had the slowest proliferation rate with the smallest number of soft agar colonies, only two. Conclusion The combination of related gene therapy is better than the use of individual gene therapy.