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目的 通过研究ＭＡＧＥ—４基因在肝细胞肝癌组织中的表达，并与患者临床资料进行分析，探讨ＭＡＧＥ—４基因与肝细胞肝癌（ＨＣＣ）患者临床指标及转移与复发的关系，为ＭＡＧＥ—４基因编码蛋白用于ＨＣＣ患者免疫治疗提供依据。方法 用ＲＴ—ＰＣＲ的方法对３１例ＨＣＣ患者癌组织及相应癌旁组织ＭＡＧＥ—４基因表达进行测定，对全部ＲＴ—ＰＣＲ扩增产物中目的基因片段进行ＤＮＡ测序以证实其为ＭＡＧＥ—４基因，患者均测定并统计ＡＦＰ、ＡＦＵ、抗ＨＣＶ、ＨＢｓＡｇ、ＡＦＰ ｍＲＮＡ、肿瘤直径等临床指标。结果 ３１例ＨＣＣ患者肝癌组织中ＭＡＧＥ－４基因表达的阳性率 ３８．７％（１２／３１）明显高于癌旁组织中 ＭＡＧＥ—４基因表达的阳性率０％（０／３１），Ｐ＜０．０１。ＨＣＣ患者肝癌组织中ＭＡＧＥ—４基因表达的阳性率与患者ＡＦＰ、ＡＦＵ、抗ＨＣＶ、ＨＢＶ标志物、ＡＦＰ ｍＲＮＡ、肿瘤直径等临床指标均无关，Ｐ＞０．０５。结论 ＭＡＧＥ－４基因在ＨＣＣ患者肝癌组织中特异高表达，可能作为ＨＣＣ患者免疫治疗攻击的靶点，ＨＣＣ患者肝癌组织中ＭＡＧＥ—４基因表达的阳性率与ＨＣＣ患者肿瘤标忘物、转移、复发均无关。
Objective To investigate the relationship between MAGE-4 gene and the metastasis and recurrence of hepatocellular carcinoma (HCC) by analyzing the expression of MAGE-4 gene in hepatocellular carcinoma (HCC) .MAGE-4 Gene encoding protein for HCC patients provide the basis for immunotherapy. Methods The expression of MAGE-4 gene in 31 cases of HCC patients and adjacent normal tissues was detected by RT-PCR. The DNA sequence of MAGE-4 gene in all the RT-PCR products was confirmed by DNA sequencing The clinical data of AFP, AFU, anti-HCV, HBsAg, AFP mRNA and tumor diameter were measured and statistically analyzed. Results The positive rate of MAGE-4 gene expression in 31 HCC patients was 38.7% (12/31), which was significantly higher than that in the adjacent non-cancerous tissues (0%, 0%, P < 0.01. The positive rate of MAGE-4 gene expression in HCC patients was not related to clinical indicators such as AFP, AFU, anti-HCV, HBV markers, AFP mRNA and tumor diameter, P> 0.05. Conclusions The MAGE-4 gene is highly expressed in HCC tissues and may play an important role in the immunotherapy of HCC. The positive rate of MAGE-4 gene in HCC patients is correlated with tumor marker, metastasis and recurrence in HCC patients Not related.