论文部分内容阅读
目的了解广东省蚊媒的构成、发生消长及西尼罗病毒的携带情况,为及时有效地切断西尼罗病毒病传播途径提供科学依据。方法 2013年5月至2014年4月在广东省的广州、佛山、东莞、茂名市的城区和郊区设点,采用灯诱法调查成蚊;一步法反转录-聚合酶链反应(RT-PCR)技术检测蚊媒携带的西尼罗病毒。结果广东省蚊媒平均密度为11.80只/灯,其密度高峰在4-5月,生境中以牲畜棚中的密度最高。致乏库蚊/致倦库蚊占捕蚊总数的89.90%,为优势蚊种。RT-PCR法未检测到西尼罗病毒阳性。结论致乏库蚊/致倦库蚊是广东省的优势蚊种,加强监测可以掌握其消长规律,为制定适宜的防制措施和预报预警其可能传播的西尼罗病毒提供科学依据;对广东省内西尼罗热的传播媒介种类及其传播流行风险有待进一步研究。
Objective To understand the composition, occurrence and growth of mosquito vectors and the carrying status of West Nile virus in Guangdong Province, and to provide a scientific basis for timely and effectively cutting off the transmission route of West Nile virus. Methods From May 2013 to April 2014, mosquitoes were investigated by light-induced method in the urban and suburban areas of Guangzhou, Foshan, Dongguan and Maoming in Guangdong Province. RT-PCR was used to detect adult mosquitoes. PCR) technique to detect West Nile virus carried by mosquito vectors. Results The average mosquito density in Guangdong Province was 11.80 / lamp, with the highest density in April-May and the highest density in livestock shed. Culex pipiens pallens / Culex pipiens quinquefasciatus accounted for 89.90% of the total number of mosquitoes, the dominant mosquito species. West Nile virus positive was not detected by RT-PCR. Conclusion Culex pipiens pallens and Culex pipiens quinquefasciatus are the dominant mosquito species in Guangdong Province. Monitoring can control their growth and decline and provide a scientific basis for formulating appropriate control measures and predicting and warning the possible transmission of West Nile virus. West Nile fever in the province of the types of media and the spread of epidemic risks to be further studied.