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在最近20年中测定胰岛细胞抗体(ICA)和胰岛素自身抗体(IAA)已成为识别胰岛素依赖型糖尿病(IDDM)的基本方法,但利用ICA和IAA诊断IDDM的敏感性和特异性均较差。近年研究表明谷氨酸脱羧酶抗体(GAA)是IDDM诊断的敏感和特异标志。本研究采用基因重组人CAD_(65)为抗原的间接ELISA法检测DM患者GAA,探讨该检测方法对DM诊断的临床意义。1 对象和方法1.1 对象 ①健康对照组:选择经体检和常规化验无异常的健康人31例(男11例,女20例),平均年龄35(5～68)岁,无糖尿
The determination of islet cell antibodies (ICA) and insulin autoantibodies (IAA) in the last 20 years has become a basic method of identifying insulin-dependent diabetes mellitus (IDDM), but the diagnostic and diagnostic specificity of IDA using both ICA and IAA is poor. Recent studies have shown that glutamic acid decarboxylase antibody (GAA) is a sensitive and specific marker for the diagnosis of IDDM. In this study, GAA was detected by indirect ELISA in patients with DM using recombinant human CAD_ (65) as antigen, and the clinical significance of this detection method in the diagnosis of DM was discussed. 1 Subjects and Methods 1.1 Subjects ① healthy control group: 31 healthy subjects (11 males and 20 females) were selected who had no abnormalities by physical examination and routine test, with an average age of 35 (5-68) years, with no diabetes