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随着纳米材料在组织工程和再生医学中越来越多的应用,干细胞和纳米材料之间的相互作用成为关键环节,而纳米颗粒进入细胞是关系到纳米材料安全性和干细胞命运调控的首要问题.干细胞传代是其应用中必不可少的过程,但关于传代代数对干细胞摄取纳米颗粒的影响的研究还较少.此外,干细胞和纳米材料的相互作用还与纳米颗粒的存在方式有关.本文在玻璃片上组装了条带状的γ-Fe_2O_3纳米颗粒组装结构,并在该表面上培养SD大鼠脂肪间充质干细胞,然后研究了不同代数的干细胞对组装和游离的纳米颗粒的吞噬情况.结果发现,随着细胞代数增加,细胞活力降低,细胞衰老水平增加,并且细胞膜的表面呈现出粗糙和不均匀的形貌.当与游离的γ-Fe_2O_3纳米颗粒共培养时,细胞内铁含量随着代数的增加而减少,但在组装体上培养的不同代数的细胞具有相似的铁含量,并且胞内铁含量极少.另外,磁感应蛋白的表达表明磁性纳米颗粒的组装体对细胞有磁效应.该研究表明,细胞代数的选择对研究颗粒内化实验是至关重要的,细胞代数应该作为细胞摄取实验的一个重要考虑因素.
As nanomaterials are used more and more in tissue engineering and regenerative medicine, the interaction between stem cells and nanomaterials becomes a key issue. The entry of nanoparticles into the cell is the primary issue that concerns the safety of nanomaterials and the regulation of stem cell fate. The passage of stem cells is an indispensable process for its application, but there is less research on the effect of passage algebra on the uptake of nanoparticles by stem cells.In addition, the interaction between stem cells and nanomaterials is also related to the existence of nanoparticles.In this paper, The assembly of ribbon-shaped γ-Fe_2O_3 nanoparticles was assembled on the surface of SD rat adipose tissue-derived mesenchymal stem cells, and the phagocytosis of assembled and free nanoparticles by different generations of stem cells was studied. , With the cell algebra increased, cell viability decreased, cell senescence increased, and the cell membrane surface showed a rough and uneven morphology.When co-cultured with free γ-Fe_2O_3 nanoparticles, intracellular iron content with the algebra But the algebraic cells cultured on the assembly had similar iron content, and the cells In addition, the expression of magnetic sensing proteins indicates that magnetic nanoparticle assemblies have a magnetic effect on the cells.The study shows that the choice of cell algebra is crucial for the study of particle internalization experiments, and cell algebra should be used as a cell An important consideration when taking an experiment.