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用已构建的不同载体乙型肝炎s基因疫苗 (pCR3.1 S ,pcDNA3 S)和乙型肝炎C基因疫苗(pCR3.1 C)分别给Balb/c小鼠多点肌肉注射 ,2周后追加免疫一次 ,用ELISA法及MTT法分别检测小鼠血清抗 HBs、抗 HBc抗体及脾细胞对HBsAg或HBcAg的特异性增殖反应。结果 ,免疫接种 2周后小鼠血清抗 HBs及抗 HBc抗体OD值分别为 0 .8373和 0 .7961均明显高于对照组 (0 .1 2 56) ,载体 pCR3.1 S的表达效力稍高于 рсDNA3 S ,但同一基因不同载体间无显著差异。不同靶基因血清抗体滴度及脾细胞对HBsAg或HBcAg的刺激指数均明显高于对照组 ,pCR3.1 C组刺激指数 (1 .935)明显高于 pCR3.1 S组 (1 .658)。结果提示 ,HBVS和C基因疫苗均可诱导较强的体液和细胞免疫应答强度 ;C基因以细胞免疫增高为主
Balb / c mice were intramuscularly injected with different vectors of hepatitis B virus (pCR3.1S, pcDNA3S) and hepatitis C gene vaccine (pCR3.1C). Two weeks later, After immunization, the anti-HBs, anti-HBc antibodies and splenocytes of mice were detected by ELISA and MTT respectively for the specific proliferative responses to HBsAg or HBcAg. As a result, the serum anti-HBs and anti-HBc antibody OD values of mice after 2 weeks of immunization were 0.8373 and 0.7961, respectively, which were significantly higher than those of the control group (0.12 56). The expression level of pCR3.1 S Higher than рсDNA3 S, but no significant difference between different vectors of the same gene. The serum antibody titers of different target genes and the spleen cells stimulated index of HBsAg or HBcAg were significantly higher than that of the control group. The stimulation index of pCR3.1 C group (1. 935) was significantly higher than that of pCR3.1 S group (1.658). The results suggest that HBV and C gene vaccines can induce strong humoral and cellular immune response intensity; C gene to cellular immunity increased mainly