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目的观察Nogo-66受体(NgR)mRNA在成年大鼠视神经的表达并探讨其意义。方法取8只成年大鼠视神经及坐骨神经,将拟杂交的组织切片分为3组:视神经实验组、坐骨神经对照组、视神经阴性对照组。采用原位杂交技术观察NgRmRNA在视神经和坐骨神经中的表达。结果大鼠视神经切片中NgRmRNA均表达阳性,阳性信号沿视神经长轴排列;所有大鼠坐骨神经切片中NgRmRNA的表达均为阴性。结论在成年大鼠视神经中NgRmRNA广泛表达,而坐骨神经中不表达,提示NgR阳性表达及分布与视神经再生能力低下密切相关。
Objective To observe the expression of Nogo-66 receptor (NgR) mRNA in the optic nerve of adult rats and its significance. Methods The optic nerve and sciatic nerve of 8 adult rats were taken and divided into three groups: optic nerve experimental group, sciatic nerve control group and optic nerve negative control group. In situ hybridization was used to observe the expression of NgR mRNA in optic nerve and sciatic nerve. Results NgR mRNA was positive in optic nerve slices of rats, and the positive signals were arranged along the long axis of optic nerve. The expression of NgR mRNA in sciatic nerve slices of all rats was negative. Conclusion NgR mRNA is widely expressed in the optic nerve of adult rats, but not in the sciatic nerve, suggesting that the positive expression and distribution of NgR is closely related to the low ability of optic nerve regeneration.