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目的 比较采用蒸馏水和空白管调零点测定家兔全血胆碱酯酶活力的差异 ,从而改进药理实验方法。方法 实验共用家兔 82只 ,每次用 1只各组分别用蒸馏水和空白管调零点 ,来测定并计算家兔给药前和中毒时的胆碱酯酶活力 ,收集数据并进行统计分析。结果 用蒸馏水和空白管调零点测定家兔全血胆碱酯酶活力 ,两者无显著性差异 ,(P >0 .0 5 )。结论 可以直接用空白管调零点测定全血胆碱酯酶活力 ,以简化实验操作步骤和计算公式
Objective To compare the difference of cholinesterase activity in whole blood of rabbits by using zero point of distilled water and blank tube to improve the pharmacological experimental method. Methods A total of 82 rabbits were used in this experiment. Cholinesterase activity was measured and calculated before and after poisoning in rabbits by using distilled water and blank tube. The data were collected and statistically analyzed. Results The whole blood cholinesterase activity in rabbits was determined by zero point adjustment of distilled water and blank tube. There was no significant difference between the two groups (P> 0.05). Conclusion Blank tube can be used directly to determine the whole blood cholinesterase activity in order to simplify the experimental procedures and calculation formula