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从黄瓜叶片中分离获得1个丙二烯氧化物环化酶基因,命名为CsAOC3。该基因c DNA全长为753 bp,编码250个氨基酸,与甜瓜的丙二烯氧化物环化酶(AOC)序列同源性最高。通过荧光定量PCR(qRT-PCR)分析发现,CsAOC3在低氮胁迫条件下只有在耐低氮黄瓜品系D0328中的表达量明显上调,在耐低氮能力弱的黄瓜品系D0422中的表达量并无明显变化。研究结果可为黄瓜耐低氮的基因功能研究提供理论依据。
One allene cyclase gene was isolated from cucumber leaves and named as CsAOC3. The gene cDNA was 753 bp in length and encoded a polypeptide of 250 amino acids with the highest homology with the allelochemical cyclase (AOC) sequence of melon. Quantitative PCR (qRT-PCR) analysis showed that CsAOC3 was only up-regulated in low-nitrogen-resistant cucumber line D0328 under low nitrogen stress, but not in low-nitrogen-tolerant cucumber line D0422 obvious change. The results provide a theoretical basis for the study of gene function of cucumber with low nitrogen tolerance.