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目的 研究多西紫杉醇对骨肉瘤细胞生长抑制及诱导凋亡作用。方法 应用细胞计数、形态学观察、蛋白含量测定、流式细胞术和电镜等方法对多西紫杉醇诱导的骨肉瘤进行检测和观察。结果 多西紫杉醇在浓度为 4μg/ml时 ,对骨肉瘤细胞有明显的生长抑制作用 ,并且存在着时间依赖关系和一定范围内的剂量依赖关系。多西紫杉醇可将骨肉瘤细胞阻断于G1期 ,并诱导凋亡 ,其凋亡细胞具有典型凋亡细胞特征。用流式细胞仪测定细胞周期 ,可见明显的凋亡峰。电镜观察染色质沿皱缩的核膜下凝聚 ,细胞表面出现凋亡小体 ,基质呈絮状致密化。在紫杉醇短时间作用去除后再培养比持续作用更易促使细胞凋亡。同时 ,多西紫杉醇对荷瘤小鼠也有明显的抑制作用。结论 多西紫杉醇对骨肉瘤细胞的细胞毒作用是诱导其凋亡的结果 ,这种诱导凋亡的能力是紫杉醇疗效的基础 ,在对骨肉瘤的治疗中 ,紫杉醇有着巨大的潜力。
Objective To study the inhibitory effect of docetaxel on the growth inhibition and apoptosis of osteosarcoma cells. Methods Docetaxel-induced osteosarcoma was detected and observed by cell counting, morphological observation, protein content determination, flow cytometry and electron microscopy. Results At a concentration of 4 μg/ml, docetaxel had significant growth inhibitory effects on osteosarcoma cells, and there was a time-dependent and dose-dependent relationship. Docetaxel can block osteosarcoma cells in G1 phase and induce apoptosis. The apoptotic cells have typical apoptotic characteristics. Flow cytometry was used to determine the cell cycle and obvious apoptosis peaks were observed. Electron microscopy revealed that the chromatin condensed along the collapsed nuclear membrane and apoptotic bodies appeared on the cell surface, and the matrix was flocculated and densified. After paclitaxel was removed for a short period of time, the culture was more likely to induce apoptosis than the sustained effect. At the same time, docetaxel has obvious inhibitory effects on tumor-bearing mice. Conclusion The cytotoxic effect of docetaxel on osteosarcoma cells is the result of its induction of apoptosis. This ability to induce apoptosis is the basis of paclitaxel efficacy. Paclitaxel has great potential in the treatment of osteosarcoma.