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‘桂林马蹄’和‘余杭大红袍’荸荠试管球茎形成的最适培养基分别为ＭＳ＋６ＢＡ１．０ ｍｇ／Ｌ＋ 蔗糖９０ ｇ／Ｌ和ＭＳ＋ ６ＢＡ０ ．８ ｍｇ／Ｌ＋ 蔗糖９０ ｇ／Ｌ， 最适培养条件为１８ ℃、光照８ ｈ／ｄ。要达到８５ ％ 左右的发芽率，两品种分别需鲜重为０ ．３ 或０ ．２ ｇ 以上的试管球茎。球茎风干至鲜重的９０ ％ 后贮藏在玻璃瓶内，０ ～１５ ℃黑暗条件下保存６ 个月后发芽率可达９０％ 以上。
The optimal culture mediums for the formation of bulblets of ’Guilin Horseshoe’ and ’Yuhang Dahongpao’ were respectively MS + 6BA1.0 mg / L + sucrose 90 g / L and MS + 6BA0. 8 mg / L + sucrose 90 g / L, the optimum culture conditions were 18 ℃, light 8 h / d. To reach about 85% germination rate, respectively, two varieties of fresh weight to 0. 3 or 0. 2 g or more test tube bulb. Bulbs dried to fresh weight 90% after storage in glass bottles, 0 ~ 15 ℃ dark conditions after 6 months of germination rate of up to 90%.