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目的探讨二氢二醇脱氢酶(DDI)在前列腺癌中的表达及意义。方法应用逆转录聚合酶链式反应方法检测DD亚型之—DDI在前列腺癌组织(11例)及正常前列腺组织(10例)中的表达水平。应用密度扫描技术半定量分析RT-PCR产物电泳条带的密度。结果DDImRNA在前列腺癌组织中表达明显增高,吸光度比值0．184～0．478,中位数为0．289：在正常前列腺组织中的表达水平较低,吸光度比值0．590～1．216,中位数为0．801。癌与正常前列腺组织间DDImRNA的表达差异有显著性(P＜0．001)。结论DDImRNA在前列腺癌中的高表达提示DD可能在前列腺癌的发生发展中发挥着重要的作用。
Objective To investigate the expression of dihydrodiol dehydrogenase (DDI) in prostate cancer and its significance. Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression level of DD in prostate cancer tissue (11 cases) and normal prostate tissue (10 cases). Semi-quantitative analysis of density of electrophoresis bands of RT-PCR products was performed using density scanning technique. Results The expression of DDImRNA in prostate cancer tissues was significantly higher than that in normal prostate tissues, the absorbance ratio was 0.184 ~ 0.478, the median was 0.289. The expression level of DDImRNA in normal prostate tissue was low, the absorbance ratio was 0.590 ~ 1.216, The median was 0.801. There was a significant difference in the expression of DDImRNA between cancer and normal prostate tissue (P <0.001). Conclusion The high expression of DDImRNA in prostate cancer suggests that DD may play an important role in the development of prostate cancer.