目的:研究猪胎盘水提物(PPE)对小鼠的抗氧化及学习记忆调节作用。方法:将经D-半乳糖诱导成功的32只衰老小鼠随机分成PPE高、低剂量组、阳性对照组、模型组,分别以PPE 30,20 mg·kg~(-1)·d~(-1)、维生素E 20 mg·kg~(-1)·d~(-1)、0.9%Na Cl溶液20 m L·kg~(-1)·d~(-1)经皮给药,另取8只正常小鼠作为正常对照(0.9%Na Cl溶液20 m L·kg~(-1)·d~(-1)),连续给药6周。给药后每周称量小鼠体重。停药前1周开始采用Morris水迷宫法检测小鼠学习记忆能力。第6周末处死小鼠,取腹部皮肤及眼球取血,测量超氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(Hyp)的含量,并观察病理切片。结果:与正常组相比,模型组小鼠水迷宫找到平台所需时间明显延长,小鼠在平台象限内停留时间、停留时间百分比、穿越平台次数均显著降低(P<0.05)。模型组小鼠体重、SOD含量、MDA含量、Hyp含量较正常组均有显著差异(P<0.01,P<0.05),皮肤表皮层不规则,真皮层皱缩,胶原纤维排列紊乱无序。经过PPE治疗后,高、低剂量组小鼠水迷宫找到平台时间明显缩短,小鼠在平台象限内停留时间、停留时间百分比、穿越平台次数均增高;高、低剂量组的体重、SOD含量、Hyp含量均明显上升(P<0.01,P<0.05),MDA含量明显下降(P<0.01,P<0.05);PPE高、低剂量组小鼠的皮肤结构较完整,表皮层规则,真皮层厚度增加,胶原纤维排列整齐。结论:猪胎盘水提物具有抗氧化及改善小鼠记忆力的作用。 Objective: To study the anti-oxidation and learning and memory effects of porcine placenta water extract (PPE) on mice. Methods: Thirty-two aging mice induced by D-galactose were randomly divided into high and low dose PPE group, positive control group and model group. The rats in each group were treated with PPE 30, 20 mg · kg -1 · d ~ (-1) -1), vitamin E 20 mg · kg -1 · d -1, 0.9% NaCl 20 mg · kg -1 · d -1, Another 8 normal mice were used as normal control (0.9% NaCl solution 20 m L · kg -1 d -1) for 6 weeks. Mice were weighed weekly after dosing. One week before discontinuation, Morris water maze test was used to detect learning and memory in mice. At the end of the 6th week, the mice were sacrificed and the skin of the abdomen and the eyeballs were taken for blood. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and hydroxyproline (Hyp) were measured and pathological sections were observed. Results: Compared with the normal group, the time required to find the platform by the water maze in the model group was significantly longer, and the retention time, the percentage of residence time and the number of crossing the platform in the platform quadrant were significantly decreased (P <0.05). The body weight, SOD content, MDA content and Hyp content in the model group were significantly different from those in the normal group (P <0.01, P <0.05). The skin epidermis was irregular, the dermis was collapsed and the disordered collagen fibers arranged. After PPE treatment, the water maze of mice in the high and low dose groups found that the time of finding the platform was significantly shortened, the duration of the mice in the platform quadrant, the percentage of residence time and the number of crossing the platform were all increased; the body weight, SOD content, Hyp content was significantly increased (P <0.01, P <0.05), MDA content was significantly decreased (P <0.01, P <0.05); PPE high and low dose group of mice skin structure is more complete, the rules of the epidermis, dermis thickness Increase collagen fibers arranged in neat rows. Conclusion: The water extract of porcine placenta has the effect of anti-oxidation and improving the memory of mice.