论文部分内容阅读
为挖掘控制水稻千粒质量的数量性状位点(QTL),同时为水稻超高产育种提供重要的育种材料,利用大粒型水稻材料lg1与常规籼稻品种9311构建的F2代遗传分离群体,采用完备区间作图法(ICIM),以LOD值2.5为阀值,对水稻千粒质量QTLs进行检测、分析。结果表明,F2代群体中千粒质量性状呈连续变异的单峰分布,为多基因控制的数量性状;共检测到千粒质量QTLs 5个,分布于第2、5、9号染色体上,LOD值介于2.65~11.77之间,表型贡献率变异范围为4.62%~52.78%,其中表型贡献率大于10%的主效QTLs共有3个;除q TGW-2-1等位基因来源于9311外,其余4个QTLs增效等位基因均来自大粒材料lg1。定位的QTLs所在区间均有相关QTLs或基因被报道,是否为等位基因则需进一步试验验证。研究结果为大粒材料lg1千粒质量QTLs的精细定位及其在水稻超高产育种中的应用奠定了基础。
In order to excavate the quantitative trait locus (QTL) for controlling the quality of rice grain and to provide important breeding material for super-high-yielding rice breeding, the genetic segregation population F2 was constructed using large grain type rice material lg1 and conventional indica variety 9311, Mapping method (ICIM), the LOD value of 2.5 as a threshold, the quality of rice grain QTLs were detected and analyzed. The results showed that the QTLs of 1000-grain quality in F2 population showed a continuous and unimodal distribution and were quantitative traits controlled by multiple genes. Five QTLs of 1000-grain quality were detected on chromosomes 2, 5 and 9, and LOD The value of phenotypic contribution ranged from 4.62% to 52.78%. Among them, there were 3 major QTLs whose phenotypic contribution rate was more than 10%. Except q TGW-2-1 allele was derived from 9311, the other four QTLs synergistic alleles were from large grain material lg1. All QTLs or genes related to QTLs located in the QTLs were reported. Whether the QTLs are alleles or not is subject to further experimental verification. The results provide the basis for the fine mapping of 1000-grain QTLs for large grain materials and their application in super-high-yielding rice breeding.