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我们采用引物R200和R300，对新疆克拉玛依地区皮肤利什曼病（CL）患者的皮肤病变组织内抽提的微量利什曼原虫SSUrDNA，以及有关利什县原虫种株的SSUrDNA进行PCR扩增，然后分别采用限制住内切酶Rsal和Hhal对PCR扩增产物进行限制性酶切分析。结果显示：采用RsaⅠ进行限制性酶切分析，克拉玛依地区2例CL患者皮肤病变组织标本的PCR扩增产物经酶切后，其电泳图形与L．tropica完全相同。显示克拉玛依地区CL病原体SSUrDNA的PCR扩增产物与L．tropica存在相同的限制性内切酶图谱。
We used primers R200 and R300 to amplify SSUrDNA of leishmania minor leishmanii (SSM) DNA extracted from skin lesions of skin leishmaniasis (CL) patients in Karamay, Xinjiang, and SSUrDNA of Leishmania isolates. Restriction enzyme digestion analysis of PCR amplification products was performed using restriction endonuclease Rsal and Hhal respectively. The results showed that using Rsa Ⅰ restriction enzyme digestion analysis, two cases of CL patients in Karamay skin lesions of the PCR products amplified by enzyme digestion, the electrophoresis patterns and L. Tropica exactly the same. The PCR products and CL of CL gene of CL pathogens in Karamay region were displayed. The same restriction enzyme map exists for tropica.