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本研究以多年筛选出的东北霜霉菌Ｐｅｒｏｎｏｓｐｏｒａｍａｎｓｃｈｕｒｉｃａ（Ｎａｕｍ．）Ｓｙｄ．为诱导菌，在黄瓜植株的３～４叶期，采用茎部注射、喷雾和灌根等三种诱导接种方法，诱导植株产生诱导抗性，测定东北霜霉菌对黄瓜霜霉病的免疫作用。研究结果表明：东北霜霉菌免疫作用的强弱与诱导接种方法有关。在３种诱导接种方法中，以注茎诱导接种法的免疫作用最强，使黄瓜植株产生的系统保护作用最明显，表现为黄瓜霜霉病的发病始期较对照株向后推迟１２～１３天，平均病情指数降低１７．６；喷雾诱导接种的免疫作用较弱；而灌根诱导接种法，未能反应出东北霜霉菌的免疫作用
In this study, we selected Peronosporamanschurica (Naum.) Syd from Northeast China for many years. As the induced strain, three induction inoculation methods such as stem injection, spray and irrigation were used to induce induced resistance in cucumber plants at 3 ~ 4 leaf stage. The anti-fungal effects of northeastern downy mildew on cucumber downy mildew were measured. The results showed that the strength and weakness of northeastern downy mildew inoculation were related to the inoculation method. In the three kinds of inoculation methods, the injection of stem induction of vaccination strongest immune effect, the cucumber plants produce the most obvious system protection, showing the beginning of the onset of cucumber downy mildew delayed 12 to 13 days later than the control strain , The average disease index decreased by 17.6; the immunization effect of spray-induced vaccination was weak; however, the induction by inoculation method failed to reflect the immunogenicity of northeastern downy mildew