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目的观察连接蛋白36(Cx36)在癫持续状态大鼠海马神经元的表达。方法建立36只氯化锂-匹罗卡品诱导的癫持续状态大鼠模型,随机分为生理盐水组、喹啉组、辛醇组(每组12只),分别予以腹腔注射生理盐水、喹啉、辛醇,通过Racine评分判断大鼠给药前后癫发作情况,利用免疫荧光染色法、Western-blot法检测各组大鼠海马Cx36的表达。结果喹啉组和辛醇组给药前后大鼠行为学评分比较差异有统计学意义(P<0.01);而生理盐水组差异无统计学意义(P>0.05)。喹啉组和辛醇组大鼠海马神经元Cx36的表达明显低于生理盐水组(P<0.01),但2组Cx36的表达量差异无统计学意义(P>0.05)。结论 Cx36在癫发作中起着重要作用,可能成为潜在的治疗靶点。
Objective To investigate the expression of connexin 36 (Cx36) in hippocampal neurons of rats with epileptic seizure. Methods Thirty-six lithium-pilocarpine-induced epileptic rats were established and randomly divided into normal saline group, quinoline group and octanol group (n = 12 each). The rats were injected intraperitoneally with normal saline, Quinoline and octanol were used to determine the onset of epilepsy in rats before and after administration. The expression of Cx36 in hippocampus was detected by immunofluorescence and Western-blot. Results Before and after administration, the behavioral scores of quinoline group and octanol group were significantly different (P <0.01), but there was no significant difference between the two groups (P> 0.05). The expression of Cx36 in hippocampal neurons of quinoline group and octanol group was significantly lower than that of saline group (P <0.01), but the expression of Cx36 in two groups had no significant difference (P> 0.05). Conclusion Cx36 plays an important role in epileptic seizures and may be a potential therapeutic target.