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目的:探讨KISS1(KiSS-1 metastasis-suppressor)和骨桥蛋白(osteopontin,OPN)在上皮性卵巢癌(epithelial ovariancancer,EOC)组织中的表达及其临床意义。方法:选取2009年3月至2010年10月在河北医科大学第四医院妇科接受手术的上皮性卵巢肿瘤患者组织标本67例,免疫组化法检测KISS1和OPN在肿瘤组织中的表达,分析其相关性和临床意义。结果:KISS1蛋白在EOC组织中的表达明显低于其在卵巢良性肿瘤组织中的表达[39.5%(17/43)vs 75.0%(18/24);χ2=7.765,P=0.005];有淋巴结转移组中KISS1的表达低于无淋巴结转移组[25.0%(7/28)vs 66.7%(10/15);χ2=7.094,P=0.008];在不同临床分期组中,Ⅰ+Ⅱ期EOC中KISS1的表达高于Ⅲ+Ⅳ期[61.1%(11/18)vs 24.0%(6/25);χ2=6.029,P=0.014]。OPN蛋白在EOC组织中的表达率明显高于其在卵巢良性肿瘤组织中的表达[74.4%(32/43)vs 37.5%(11/24);χ2=5.475,P=0.019];在有淋巴结转移组中OPN的表达高于无淋巴结转移组[89.3%(25/28)vs 46.7%(7/15);χ2=7.251,P=0.007];在不同临床分期组中,Ⅰ+Ⅱ期EOC中OPN的表达低于Ⅲ+Ⅳ期[50.0%(9/18)vs 92.0%(23/25);χ2=7.616,P=0.006]。KISS1和OPN蛋白的表达与EOC的病理类型及患者年龄无关(P>0.05)。在EOC中,KISS1与OPN蛋白的表达呈负相关(r=-0.507,P=0.001)。结论:KISS1和OPN可能参与了EOC的发生、发展和转移,有可能成为EOC预后判断的生物学标志物。
Objective: To investigate the expression of KISS1 (KiSS-1 metastasis-suppressor) and osteopontin (OPN) in epithelial ovarian cancer (EOC) tissues and their clinical significance. Methods: From March 2009 to October 2010, we collected 67 cases of epithelial ovarian tumor patients undergoing gynecological surgery in the Fourth Hospital of Hebei Medical University from March 2009 to October 2010 and detected the expression of KISS1 and OPN in the tumor tissue by immunohistochemistry Relevance and clinical significance. Results: The expression of KISS1 protein in EOC tissues was significantly lower than that in benign ovarian tumors (39.5% (17/43) vs 75.0% (18/24), χ2 = 7.765, P = 0.005] The expression of KISS1 in the metastasis group was lower than that in the group without lymph node metastasis [25.0% (7/28) vs 66.7% (10/15); χ2 = 7.094, P = 0.008] The expression of KISS1 was higher than that of stage Ⅲ + Ⅳ [61.1% (11/18) vs 24.0% (6/25); χ2 = 6.029, P = 0.014]. The expression of OPN protein in EOC tissues was significantly higher than that in OPN tissues [74.4% (32/43) vs 37.5% (11/24); χ2 = 5.475, P = 0.019] The expression of OPN in the metastasis group was higher than that in the group without lymph node metastasis [89.3% (25/28) vs 46.7% (7/15); χ2 = 7.251, P = 0.007] The expression of OPN was lower than that of stage Ⅲ + Ⅳ [50.0% (9/18) vs 92.0% (23/25); χ2 = 7.616, P = 0.006]. The expression of KISS1 and OPN protein had no correlation with pathological type and age of patients (P> 0.05). In EOC, there was a negative correlation between KISS1 and OPN protein expression (r = -0.507, P = 0.001). Conclusion: KISS1 and OPN may participate in the occurrence, development and metastasis of EOC, which may become the biomarker of prognosis of EOC.