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为了确定大鼠的体重是否在标准气压缺氧环境下会减轻,本实验将12只5周龄Wis-tar大鼠移高至海拔2260m并使之持续暴露于含10%O2的低氧小低压舱内(相当于在海拔5000m)20日。在基线每5日测一次体重。由于标准气压缺氧环境可影响血循环功能,所以在大鼠移入高原前后20天,分别对其肺动脉压和血红蛋白浓度进行了测定。基线处体重为(222.4±20.7)g,而与基线处相比。大鼠体重在移高5、10、15和20天后明显下降,分别下降了(4.88±SD)%、(7.67土SD)%、(11.05±SD)%和(11.9±SD)%,差异显著(P<0.05)。基线处大鼠的平均肺动脉压为(21.3±2.5)mmHg,低氧暴露20天后肺动脉压明显增高[(45.3±5.8)mmHg,P<0.001]。血红蛋白浓度在基线处的平均值为(14.5±3.2)g/L,而在低氧暴露20天后明显增加[(22.6±5.4)g/L,P<0.001]。该结果显示,模拟高海拔可使大鼠体重明显下降,这与先前报道的人体试验结果相似。结果也进一步说明,标准气压下持续的低氧环境对肺动脉压和血红蛋白浓度可产生实质性影响。
In order to determine whether the body weight of rats decreased under standard pressure and hypoxia, Twelve 5-week-old Wis-tar rats were elevated to an altitude of 2260 m and exposed continuously to hypoxia with 10% O2 Cabin (equivalent to 5000m above sea level) 20 days. Body weight is measured every 5 days at baseline. Because standard aerobic hypoxia can affect blood circulation, the pulmonary arterial pressure and hemoglobin concentration were measured respectively 20 days before and after the rat moved into the plateau. At baseline, body weight was (222.4 ± 20.7) g, compared with baseline. The body weight decreased significantly after 5, 10, 15 and 20 days and decreased by (4.88 ± SD)%, (7.67 ± SD)%, (11.05 ± SD)% and (11.9 ± SD)%, respectively (P <0.05). At baseline, the mean pulmonary arterial pressure was (21.3 ± 2.5) mmHg in rats, and pulmonary arterial pressure was significantly increased 20 days after hypoxia exposure (45.3 ± 5.8 mmHg, P <0.001). Hemoglobin concentration was (14.5 ± 3.2) g / L at baseline and significantly increased [(22.6 ± 5.4) g / L, P <0.001] after 20 days of hypoxia exposure. The results show that simulating high altitude can significantly reduce body weight in rats, which is similar to previously reported human test results. The results further demonstrate that a sustained hypoxic environment at standard pressure can have a substantial effect on pulmonary arterial pressure and hemoglobin concentration.