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试验首先研究山野豌豆不同生长时期的营养成分含量及干物质产量,接着利用体外发酵试验来研究其对瘤胃体外发酵和甲烷产量的影响。分别于2014年6月、7月、8月和9月初在长白山地区采集山野豌豆,烘干后测其产量与各营养成分含量;选用3头生长状况相似,体质量均在450 kg左右的延边黄牛,采集瘤胃液进行体外发酵试验。结果表明:山野豌豆蛋白质产量在8月份达到最大,与相对饲用价值(RFV)曲线相交于8月初,此时植株干物质蛋白含量在18.37%以上;与6月和9月份收获的山野豌豆相比,7月和8月收获的山野豌豆显著提高体外发酵培养液的p H(P<0.05),降低NH_3-N含量(P<0.05),减少总产气量和甲烷产量(P<0.05),降低各挥发性脂肪酸(VFA)浓度(P<0.05)。综上所述,山野豌豆是极具饲用价值的含单宁牧草,且在8月初利用价值最高,能够优化瘤胃体外发酵过程,降低甲烷的排放量。
The experiment first studied the nutrient content and dry matter yield of wild pea in different growth stages, and then used in vitro fermentation test to study its effect on rumen in vitro fermentation and methane production. The wild peas were harvested in Changbai Mountain area in June, July, August and early September, respectively, and their contents and contents of nutrients were measured after drying. Three peanut plants with similar growth status and weighing about 450 kg Cattle, collecting rumen fluid for in vitro fermentation test. The results showed that the protein yield of mountain pea reached its maximum in August and crossed with the relative feed value (RFV) curve at the beginning of August when the dry matter protein content of the plant was above 18.37%. Compared with the harvest of mountain pea in June and September (P <0.05), decreased the content of NH_3-N (P <0.05), decreased the total gas production and the methane production (P <0.05) compared with the wild pea harvested in July and August, Reduce the concentration of volatile fatty acids (VFA) (P <0.05). In summary, the wild pea is a highly forage-containing tannin containing grass, and the highest value in early August, can optimize the in vitro fermentation process rumen, reduce methane emissions.