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目的：克隆人降钙素ｃＤＮＡ。方法和结果：应用ＡＢＩ３９１ＤＮＡ合成仪，将人降钙素ｃＤＮＡ分成６个片段合成。退火、磷酸化、连接，最终将人降钙素ｃＤＮＡ克隆于载体ｐＧＥＭ７ｚ（＋），经ＤＮＡ双链测序，证明克隆到的人降钙素ｃＤＮＡ序列与设计的完全一致。结论：本研究为寡肽ｃＤＮＡ（或基因）的化学合成与克隆提供了简捷、可靠的方法；为人的降钙素ｃＤＮＡ的高效表达奠定了基础。
Objective: To clone human calcitonin cDNA. Methods and Results: Human calcitonin cDNA was synthesized by ABI391 DNA synthesizer and divided into 6 fragments. Annealed, phosphorylated and ligated. Finally, the human calcitonin cDNA was cloned into the vector pGEM7z (+), and the sequence of the human calcitonin cDNA was confirmed by DNA double-stranded sequencing. Conclusion: This study provides a simple and reliable method for the chemical synthesis and cloning of oligopeptide cDNA (or gene), which lays the foundation for the high expression of human calcitonin cDNA.