论文部分内容阅读
A method for real time observation of photo-cleavage of stretched λDNA at single molecular level by afluorescent microscope coupled with CCD is developed. DNAmolecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the process of photocleavage and relaxation of DNAunder radiation of blue light is observed. We speculate thatthe conformation change of stretched DNA and the effect ofwater are likely to facilitate the effect of YOYO photocleav-age DNA molecules. The photocleavage effect of YOYO forstretched DNA may be useful to study DNA elasticity, cancerresearch as well as the interaction between DNA and dyes.